mouse tslp duoset elisa kit  (R&D Systems)

Journal: International Journal of Molecular Sciences

Article Title: Blockade of PAR2 Signaling by Punicalagin as a Therapeutic Strategy for Atopic Dermatitis

doi: 10.3390/ijms26188920

Figure Lengend Snippet: PCG suppresses PAR2-induced inflammatory signaling in HaCaT cells. ( A ) Representative immunoblot showing the effect of PCG on ERK1/2 and p65 phosphorylation. HaCaT cells were pretreated with various concentrations of PCG for 20 min before stimulation with 30 µM PAR2-AP. ( B , C ) Quantification of phosphorylated ERK1/2 and p65 levels. Phosphorylated ERK1/2 bands were normalized to total ERK1/2 and phosphorylated p65 (p-p65) was normalized to total p65. ( D ) Effect of PCG on PAR2-AP-induced IL-8 secretion. HaCaT cells were pretreated with PCG for 0 min and then stimulated with 30 µM PAR2-AP for 6 h. IL-8 levels in cell culture supernatants were measured by ELISA. Data are expressed as mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The levels of TSLP were measured using a mouse TSLP ELISA kit (catalog #MTLP00; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.

Techniques: Western Blot, Phospho-proteomics, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Molecular Sciences

Article Title: Blockade of PAR2 Signaling by Punicalagin as a Therapeutic Strategy for Atopic Dermatitis

doi: 10.3390/ijms26188920

Figure Lengend Snippet: PCG normalizes epidermal hyperplasia, inflammatory markers, and neuronal hypersensitivity in DNFB-induced atopic dermatitis. ( A ) Representative H&E-stained skin sections showing epidermal thickness on day 28. Scale bar = 100 µm. ( B ) Quantitative analysis of epidermal thickness measured from H&E-stained sections ( n = 5–6 mice per group). ( C ) Serum TSLP levels measured by ELISA. ND, not detected ( n = 5–6 mice per group). ( D ) Calcium imaging of dorsal root ganglion (DRG) neurons in response to PAR2-AP (5 µM) stimulation. Traces show [Ca 2+ ] i changes over time, with KCl (100 mM) used as a positive control for neuronal viability ( n ≥ 20 per group). Data are presented as mean ± SEM. *** p < 0.001 compared to DNFB group.

Article Snippet: The levels of TSLP were measured using a mouse TSLP ELISA kit (catalog #MTLP00; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Imaging, Positive Control