tslp  (R&D Systems)

Journal: Heliyon

Article Title: Pseudomonas aeruginosa exotoxin A as a novel allergen induced Non-T H 2 inflammation in a murine model of steroid-insensitive asthma

doi: 10.1016/j.heliyon.2024.e37512

Figure Lengend Snippet: Prokaryotic expression of rPEA and its serological identification in asthma patients. ( A ) The purity and apparent molecular weight of rPEA were determined by Coomassie brilliant blue. The target protein bands are located between 40 and 55 kDa. ( B ) Pie chart showing the proportion of positive and negative rPEA-sIgE in 120 asthma patients' sera. ( C ) Immunoblotting of IgE binding to rPEA using sera from six rPEA-sIgE positive patients (lanes 1–6). Samples of 2.5 μg per lane of rPEA was separated by electrophoresis on 12 % SDS-PAGE prior to transfer to PVDF membrane. Blots were incubated in patient sera (1:20) and then incubated with HRP-conjugated mouse anti-human IgE antibodies and visualized with ECL. M: molecular weight marker. BC: Blank control. NC: Negative control. (D) and (E) Specific IgE competitive ELISA assay. The rPEA-sIgE positive serum was incubated with increasing doses of rPEA ( D ) or P. aeruginosa lysate ( E ). The figure is shown as the inhibition of sIgE binding to plate-bound rPEA. The full, non-adjusted images of gels and blots are presented in .

Article Snippet: The ELISA kits used were as follows: IFN gamma Mouse Uncoated ELISA Kit (#88-7314-88, Thermo Fisher, USA), IL-4 mouse Uncoated ELISA Kit (#1210402, Dakewe, China; #88-7044-88, Thermo Fisher, USA), IL-5 mouse Uncoated ELISA Kit (#88-7054-88, Thermo Fisher, USA), IL-13 mouse Uncoated ELISA Kit (#88-7137-88, Thermo Fisher, USA), IL-17A (homodimer) Mouse Uncoated ELISA Kit (#88-7371-88, Thermo Fisher, USA), IL-1 beta Mouse Uncoated ELISA Kit (#88-7013-88, Thermo Fisher, USA), IL-23 Mouse Uncoated ELISA Kit (#88-7230-88, Thermo Fisher, USA), IL-33 Mouse Uncoated ELISA Kit (#88-7333-88, Thermo Fisher, USA), TSLP Mouse Uncoated ELISA Kit (#88-7490-88, Thermo Fisher, USA), IL-25 Mouse Uncoated ELISA Kit (#88-7002-88, Thermo Fisher, USA), IL-6 Mouse Uncoated ELISA Kit (#88-7064-88, Thermo Fisher, USA).

Techniques: Expressing, Molecular Weight, Western Blot, Binding Assay, Electrophoresis, SDS Page, Membrane, Incubation, Marker, Control, Negative Control, Competitive ELISA, Inhibition

Journal: Heliyon

Article Title: Pseudomonas aeruginosa exotoxin A as a novel allergen induced Non-T H 2 inflammation in a murine model of steroid-insensitive asthma

doi: 10.1016/j.heliyon.2024.e37512

Figure Lengend Snippet: Levels of serum immunoglobulin E and BALF cytokines from rPEA-sensitized and challenged mice. ( A ) The total IgE and rPEA-specific IgE in sera (n = 5–6). The sera were diluted with 1:10. ( B ) Detection of rPEA specific IgG1 and IgG2a in sera (n = 7). The serum was diluted with 1:10 5 . ( C ) Levels of IFN-γ, IL-4, IL-5, IL-13, IL-17A, IL-1β, IL-23, IL-33, TSLP, and IL-25 in BALF (n = 5). ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001. Bar, mean ± SEM.

Article Snippet: The ELISA kits used were as follows: IFN gamma Mouse Uncoated ELISA Kit (#88-7314-88, Thermo Fisher, USA), IL-4 mouse Uncoated ELISA Kit (#1210402, Dakewe, China; #88-7044-88, Thermo Fisher, USA), IL-5 mouse Uncoated ELISA Kit (#88-7054-88, Thermo Fisher, USA), IL-13 mouse Uncoated ELISA Kit (#88-7137-88, Thermo Fisher, USA), IL-17A (homodimer) Mouse Uncoated ELISA Kit (#88-7371-88, Thermo Fisher, USA), IL-1 beta Mouse Uncoated ELISA Kit (#88-7013-88, Thermo Fisher, USA), IL-23 Mouse Uncoated ELISA Kit (#88-7230-88, Thermo Fisher, USA), IL-33 Mouse Uncoated ELISA Kit (#88-7333-88, Thermo Fisher, USA), TSLP Mouse Uncoated ELISA Kit (#88-7490-88, Thermo Fisher, USA), IL-25 Mouse Uncoated ELISA Kit (#88-7002-88, Thermo Fisher, USA), IL-6 Mouse Uncoated ELISA Kit (#88-7064-88, Thermo Fisher, USA).

Techniques: