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mouse tslp duoset elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems mouse tslp duoset elisa kit
    Mouse Tslp Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+tslp+elisa+kit/Mouse+TSLP+DuoSet+ELISA/10__1007_slash_s10068___026___02125___9-63-36-41
    Average 93 stars, based on 22 article reviews
    mouse tslp duoset elisa kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Cutaneous Neuroimmune Interactions of TSLP and TRPV4 Play Pivotal Roles in Dry Skin-Induced Pruritus
    Article Snippet: .. The levels of TSLP and tryptase were measured using a mouse TSLP ELISA kit (catalog #MTLP00; R&D Systems, Minneapolis, MN, USA) and mouse tryptase ELISA kit (catalog #RD-TPS-Mu; Reddot Biotech Inc., Kelowna, BC, Canada), respectively. .. Additionally, the serum levels of mouse IL-13, IL-33, and TNF-α were detected using a MILLIPLEX ELISA kit (catalog #MCYTOMAG-70K; Millipore, Billerica, MA, USA).

    Article Title: Topical acidic cream prevents the development of atopic dermatitis- and asthma-like lesions in murine model.
    Article Snippet: Long-standing or repeated skin barrier damage followed by atopic dermatitis (AD) is the initial step of the atopic march that eventually progresses to respiratory allergies.. Maintenance of an acidic pH in the stratum corneum (SC) is an important factor for normal skin barrier function.. We performed this study to determine whether an oxazolone (Ox)-induced AD murine model can develop airway inflammation by topical application and nasal inhalation of a house dust mite, Dermatofagoides pteronyssinus (Dp), which is a novel ‘atopic march animal model’, and whether an acidic SC environment, made by repeated application of acidic cream, can interrupt this atopic march.

    Article Title: Impressic Acid Ameliorates Atopic Dermatitis-Like Skin Lesions by Inhibiting ERK1/2-Mediated Phosphorylation of NF-κB and STAT1
    Article Snippet: Nuclear factor-kappaB (NF-κB) luciferase reporter vector was obtained from Stratagene (Grand Island, NY, USA). .. The mouse IgE ELISA kit was from BD Biosciences (San Diego, CA, USA) and the mouse TSLP ELISA kit was purchased from R&D Systems (Minneapolis, MN, USA). .. PCR primers were custom synthesized by Bioneer Co. (Daejeon, Korea).

    Article Title: Fine Particulate Matter (PM2.5) is a Risk Factor for Dermatitis by Promoting the Expression of Thymic Stromal Lymphopoietin (TSLP) in Keratinocytes
    Article Snippet: .. Main reagents: RPMI-1640 culture medium (Gibco, 11875093), FBS (Gibco, 12483020), 0.25% trypsin-EDTA (Gibco, 25200114), m-xylene (sigma, 95670), 1,2,4-trimethylbenzene (sigma, 45996), formaldehyde solution (sigma, 252549), TPA (sigma, P1585), PM2.5 (bestowed by the Department of Environmental Health, School of Public Health, Fudan University), mouse TSLP ELISA kit (R&D, MTLP00), reverse transcription kit PrimeScriptTM (Takara, RR037A), and GoTap qPCR master mix (promega, A6002). ..

    Article Title: Cutaneous Neuroimmune Interactions of TSLP and TRPV4 Play Pivotal Roles in Dry Skin-Induced Pruritus.
    Article Snippet: .. Enzyme-Linked Immunosorbent Assay (ELISA) The levels of TSLP and tryptase were measured using a mouse TSLP ELISA kit (catalog #MTLP00; R&D Systems, Minneapolis, MN, USA) and mouse tryptase ELISA kit (catalog #RD-TPS-Mu; Reddot Biotech Inc., Kelowna, BC, Canada), respectively. .. Additionally, the serum levels of mouse IL-13, IL-33, and TNF-a were detected using a MILLIPLEX ELISA kit (catalog #MCYTOMAG-70K; Millipore, Billerica, MA, USA).

    Article Title: Antipruritic effect of ursolic acid through MRGPRX2/MrgprB2-dependent inhibition of mast cell degranulation and reduced TSLP production.
    Article Snippet: Ursolic acid (UA), a pentacyclic triterpene, exhibits diverse pharmacological effects, including potential treatment for allergic diseases.. It downregulates thymic stromal lymphopoietin (TSLP) and disrupts mast cell signaling pathways.. However, the exact molecular mechanism by which UA interferes with mast cell action remains unclear.

    Article Title: Blockade of PAR2 Signaling by Punicalagin as a Therapeutic Strategy for Atopic Dermatitis
    Article Snippet: After deparaffinization, the sections were stained with H&E or toluidine blue and examined under a Nikon Eclipse 80i microscope (Tokyo, Japan) at 100× magnification. .. The levels of TSLP were measured using a mouse TSLP ELISA kit (catalog #MTLP00; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. .. Mouse DRG neurons were collected and incubated in Neurobasal ® Medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) with 1 mg/mL collagenase type II (Worthington Biochemical, Lakewood, NJ, USA) for 40 min at 37 °C in a shaking incubator (60 rpm), followed by 40 min incubation with 2.5 mg/mL trypsin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in HBSS (Gibco, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: TLR4 antagonist suppresses airway remodeling in asthma by inhibiting the T-helper 2 response
    Article Snippet: TAK242 powder was purchased from Takeda Pharmaceutical Company (Osaka, Japan) and dissolved in dimethyl sulfoxide (DMSO) prior to use. .. A mouse TSLP ELISA kit was purchased from R&D Systems, Inc. (cat. no. MTLP00; Minneapolis, MN, USA), and IL-4, IL-13 and interferon (IFN)-γ ELISA kits were purchased from eBioscience; Thermo Fisher Scientific, Inc. (cat. nos. ..

    Reverse Transcription:

    Article Title: Fine Particulate Matter (PM2.5) is a Risk Factor for Dermatitis by Promoting the Expression of Thymic Stromal Lymphopoietin (TSLP) in Keratinocytes
    Article Snippet: .. Main reagents: RPMI-1640 culture medium (Gibco, 11875093), FBS (Gibco, 12483020), 0.25% trypsin-EDTA (Gibco, 25200114), m-xylene (sigma, 95670), 1,2,4-trimethylbenzene (sigma, 45996), formaldehyde solution (sigma, 252549), TPA (sigma, P1585), PM2.5 (bestowed by the Department of Environmental Health, School of Public Health, Fudan University), mouse TSLP ELISA kit (R&D, MTLP00), reverse transcription kit PrimeScriptTM (Takara, RR037A), and GoTap qPCR master mix (promega, A6002). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Fine Particulate Matter (PM2.5) is a Risk Factor for Dermatitis by Promoting the Expression of Thymic Stromal Lymphopoietin (TSLP) in Keratinocytes
    Article Snippet: .. Main reagents: RPMI-1640 culture medium (Gibco, 11875093), FBS (Gibco, 12483020), 0.25% trypsin-EDTA (Gibco, 25200114), m-xylene (sigma, 95670), 1,2,4-trimethylbenzene (sigma, 45996), formaldehyde solution (sigma, 252549), TPA (sigma, P1585), PM2.5 (bestowed by the Department of Environmental Health, School of Public Health, Fudan University), mouse TSLP ELISA kit (R&D, MTLP00), reverse transcription kit PrimeScriptTM (Takara, RR037A), and GoTap qPCR master mix (promega, A6002). ..



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    R&D Systems mouse tslp elisa kit
    PCG suppresses PAR2-induced inflammatory signaling in HaCaT cells. ( A ) Representative immunoblot showing the effect of PCG on ERK1/2 and p65 phosphorylation. HaCaT cells were pretreated with various concentrations of PCG for 20 min before stimulation with 30 µM PAR2-AP. ( B , C ) Quantification of phosphorylated ERK1/2 and p65 levels. Phosphorylated ERK1/2 bands were normalized to total ERK1/2 and phosphorylated p65 (p-p65) was normalized to total p65. ( D ) Effect of PCG on PAR2-AP-induced IL-8 secretion. HaCaT cells were pretreated with PCG for 0 min and then stimulated with 30 µM PAR2-AP for 6 h. IL-8 levels in cell culture supernatants were measured by <t>ELISA.</t> Data are expressed as mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.
    Mouse Tslp Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PCG suppresses PAR2-induced inflammatory signaling in HaCaT cells. ( A ) Representative immunoblot showing the effect of PCG on ERK1/2 and p65 phosphorylation. HaCaT cells were pretreated with various concentrations of PCG for 20 min before stimulation with 30 µM PAR2-AP. ( B , C ) Quantification of phosphorylated ERK1/2 and p65 levels. Phosphorylated ERK1/2 bands were normalized to total ERK1/2 and phosphorylated p65 (p-p65) was normalized to total p65. ( D ) Effect of PCG on PAR2-AP-induced IL-8 secretion. HaCaT cells were pretreated with PCG for 0 min and then stimulated with 30 µM PAR2-AP for 6 h. IL-8 levels in cell culture supernatants were measured by ELISA. Data are expressed as mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: Blockade of PAR2 Signaling by Punicalagin as a Therapeutic Strategy for Atopic Dermatitis

    doi: 10.3390/ijms26188920

    Figure Lengend Snippet: PCG suppresses PAR2-induced inflammatory signaling in HaCaT cells. ( A ) Representative immunoblot showing the effect of PCG on ERK1/2 and p65 phosphorylation. HaCaT cells were pretreated with various concentrations of PCG for 20 min before stimulation with 30 µM PAR2-AP. ( B , C ) Quantification of phosphorylated ERK1/2 and p65 levels. Phosphorylated ERK1/2 bands were normalized to total ERK1/2 and phosphorylated p65 (p-p65) was normalized to total p65. ( D ) Effect of PCG on PAR2-AP-induced IL-8 secretion. HaCaT cells were pretreated with PCG for 0 min and then stimulated with 30 µM PAR2-AP for 6 h. IL-8 levels in cell culture supernatants were measured by ELISA. Data are expressed as mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: The levels of TSLP were measured using a mouse TSLP ELISA kit (catalog #MTLP00; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.

    Techniques: Western Blot, Phospho-proteomics, Cell Culture, Enzyme-linked Immunosorbent Assay

    PCG normalizes epidermal hyperplasia, inflammatory markers, and neuronal hypersensitivity in DNFB-induced atopic dermatitis. ( A ) Representative H&E-stained skin sections showing epidermal thickness on day 28. Scale bar = 100 µm. ( B ) Quantitative analysis of epidermal thickness measured from H&E-stained sections ( n = 5–6 mice per group). ( C ) Serum TSLP levels measured by ELISA. ND, not detected ( n = 5–6 mice per group). ( D ) Calcium imaging of dorsal root ganglion (DRG) neurons in response to PAR2-AP (5 µM) stimulation. Traces show [Ca 2+ ] i changes over time, with KCl (100 mM) used as a positive control for neuronal viability ( n ≥ 20 per group). Data are presented as mean ± SEM. *** p < 0.001 compared to DNFB group.

    Journal: International Journal of Molecular Sciences

    Article Title: Blockade of PAR2 Signaling by Punicalagin as a Therapeutic Strategy for Atopic Dermatitis

    doi: 10.3390/ijms26188920

    Figure Lengend Snippet: PCG normalizes epidermal hyperplasia, inflammatory markers, and neuronal hypersensitivity in DNFB-induced atopic dermatitis. ( A ) Representative H&E-stained skin sections showing epidermal thickness on day 28. Scale bar = 100 µm. ( B ) Quantitative analysis of epidermal thickness measured from H&E-stained sections ( n = 5–6 mice per group). ( C ) Serum TSLP levels measured by ELISA. ND, not detected ( n = 5–6 mice per group). ( D ) Calcium imaging of dorsal root ganglion (DRG) neurons in response to PAR2-AP (5 µM) stimulation. Traces show [Ca 2+ ] i changes over time, with KCl (100 mM) used as a positive control for neuronal viability ( n ≥ 20 per group). Data are presented as mean ± SEM. *** p < 0.001 compared to DNFB group.

    Article Snippet: The levels of TSLP were measured using a mouse TSLP ELISA kit (catalog #MTLP00; R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.

    Techniques: Staining, Enzyme-linked Immunosorbent Assay, Imaging, Positive Control